Production and Optimization of Astaxanthin by Phaffia rhodozyma under Submerged Fermentation

Abstract

One of the main processes for creating astaxanthin, an essential pigment with industrial and pharmacological applications, is Phaffia rhodozyma fermentation. The effects of nitrogen supply and carbon source on Phaffia rhodozyma culture and astaxanthin production were examined using single factor and mixture design experiments in an effort to increase astaxanthin productivity. The design of the fermentation medium is a crucial step in the creation of any metabolite. Plackett-Burman Using design in the first phase was an effective way to determine how production and medium components will interact. Significantly, the second step's usage of Box-Behnken's Design of Response Surface Methodology was helpful in figuring out the ideal concentration of the elements that significantly affect the formation of astaxanthin and biomass. The optimized final composition of the submerged fermentation medium used by MTCC 7536 to produce biomass and astaxanthin. This indicates that the projected value is 65 and 68% valid. Since the medium is chemically specified, it might not affect further processing. Due to its simpler processing and practical features like experimental design, 3D contours, 3D surface graphs, Point prediction, etc., the Design Expert 7.0 software utilized for the optimization study was determined to be a very effective tool for optimization.